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Metabolic Engineering

  • Course level: Intermediate
  • Categories C-Science
  • Last Update 24/06/2021

Description

Metabolic engineering is an emerging field of biotechnology/bioprocess engineering which aims towards the purposeful modification of cellular (metabolic, gene regulatory, and signaling) processes/networks to achieve desirable goals such as enhanced production of metabolites including pharmaceuticals, biofuels and biochemicals, and other biotechnology products.

This course aims to provide fundamental and advanced knowledge in the development of microbial strain for bioproduction through metabolic engineers.

What Will I Learn?

  • Learn the basics of Metabolic Engineering from A to Z

Topics for this course

49 Lessons

Metabolic Engineering

01 Virtual Welcome00:00:00
02 Group Projects00:00:00
03 Intro to MFA00:00:00
04 Metabolic Databases00:00:00
05 Intro to FBA00:00:00
06 FBA Part 200:00:00
07 FBA Part 300:00:00
08 GEM Database00:00:00
09 GEM Files00:00:00
10 OptKnock00:00:00
11 Intro to 13C MFA00:00:00
12 13C MFA Part 200:00:00
13 13C MFA Part 300:00:00
14 Regulatory Modalities00:00:00
15 Positive & Negative00:00:00
16 Negative Feedback00:00:00
17 Trp Operon00:00:00
18 Feedback Resistant Enzymes00:00:00
19 RNA Polymerase Anatomy00:00:00
20 Promoters00:00:00
21 The Ribosome Binding Site00:00:00
22 The RBS Calculator00:00:00
23 Tradeoffs of translation00:00:00
24 Inducible promoters00:00:00
25 Building biosensors00:00:00
26 Biosensor details00:00:00
27 TF Cooperativity00:00:00
28 Dynamic knockdown00:00:00
29 Industrial DMC00:00:00
30 Synthetic circuits00:00:00
31 Genome editing00:00:00
32 Nucleases00:00:00
33 CRISPR Metabolic Engr00:00:00
34 Recombineering00:00:00
35 MAGE00:00:00
36 MAGE Applied00:00:00
37 Genomes for new chemistry00:00:00
38 Genomes & aldehydes00:00:00
39 Aldehyde reductases00:00:00
40 Creating a RARE genome00:00:00
41 Recoded E. coli00:00:00
42 Other recoded genomes00:00:00
43 Expanded genetic alphabet00:00:00
44 Minimal genomes00:00:00
45 Refactored genomes00:00:00
46 De novo pathways00:00:00
47 Experimental design steps00:00:00
48 Retrobiosynthesis00:00:00
49 Unnatural targets00:00:00
Metabolic Engineering
Free

Enrolment validity: Lifetime

Requirements

  • Basics of Microbiology, Biochemistry, Genetics