Genetic Engineering: Theory And Application

About Course

Genetic Engineering: Theory and Application.

In the current MOOCs course, I have put effort to briefly discuss biotechnology, its scope, and its impact on human life with several customized products. The Development of technology and generation of product has multiple steps and understanding these steps are being covered in this course with a discussion of biotechnology application at the end. By the end of this Genetic Engineering course, students will be able to understand the following aspects of biotechnology:1. Basic metabolic pathways and their regulation.2. Microbial growth kinetics with an emphasis on fermentation3. Basic molecular biology tools used in biotechnology.4. Basic methodology for product recovery and analysis.

INTENDED AUDIENCE: UG/PG/Ph.D./Scientist in industry

Week 1: Introduction and Basics of Biological System.
Week 2: Basics of Biological System
Week 3: Basics of Cloning (Part I)
Week 4: Basics of Cloning (Part II)
Week 5: Recombinant DNA Technology (Part I)
Week 6: Recombinant DNA Technology (Part II)
Week 7:Product Recovery and Purification (Part I)
Week 8: Product Recovery and Purification (Part II)
Week 9: Characterization of Isolated Products (Part I)
Week 10: Characterization of Isolated Products (Part II)
Week 11: Biotechnology in Social Welfare
Week 12:Summary & Conclusions

Course Content

Genetic Engineering: Theory and Application

Genetic Engineering: Theory and Application [Intro Video]

00:00
Lec 1: Cellular Structure (Part I)

00:00
Lec 2: Cellular Structure (Part II)

00:00
Lec 3: Cellular Structure (Part III)

00:00
Lec 4: Metabolic Reactions in Biological System

00:00
Lec 5: Growth Media For Different Expression System

00:00
Lec 6: Microbial Growth Kinetics

00:00
Lec 7: Isolation of a Gene Fragment (Part I)

00:00
Lec 8: Isolation of a Gene Fragment (Part II)

00:00
Lec 9: Isolation of a Gene Fragment (Part III)

00:00
Lec 10: Polymerase Chain Reaction

00:00
Lec 11: Molecular Tools for Cloning

00:00
Lec 12: Cloning Vectors- I

00:00
Lec 13: Cloning Vectors- II

00:00
Lec 14: DNA Delivery In Host- I

00:00
Lec 15: DNA Delivery In Host- II

00:00
Lec 16: Screening of Recombinant Clones

00:00
Lec 17: Protein Production in Host- I

00:00
Lec 18: Protein Production in Host- II

00:00
Lec 19: Protein Production in Host- III

00:00
Lec 20: Product Recovery from Host Cells

00:00
Lec 21: Basics of Chromatography (Part 1)

00:00
Lec 22: Basics of Chromatography (Part 2)

00:00
Lec 23: Ion-exchange Chromatography

00:00
Lec 24: Hydrophobic Interaction chromatography

00:00
Lec 25: Gel Filtration chromatography (Part 1)

00:00
Lec 26: Gel Filtration chromatography (Part 2)

00:00
Lec 27: Affinity Chromatography (Part 1)

00:00
Lec 28: Affinity Chromatography (Part 2)

00:00
Lec 29: Affinity Chromatography (Part 3)

00:00
Lec 30: Affinity Chromatography (Part 4)

00:00
Lec 31: Electrophoresis (Part 1)

00:00
Lec 32: Electrophoresis (Part 2)

00:00
Lec 33: Electrophoresis (Part 3)

00:00
Lec 34: Protein Sequencing

00:00
Lec 35: Spectroscopy (Part I)

00:00
Lec 36: Spectroscopy (Part II)

00:00
Lec 37: Biotechnology Applications (Part 1)

00:00
Lec 38: Biotechnology Applications (Part 2)

00:00
Lec 39: Biotechnology Applications (Part 3)

00:00
Lec 40: Summary and Conclusions (Part 1)

00:00
Lec 41: Summary and Conclusions (Part 2)

00:00

Student Ratings & Reviews

No Review Yet

About Course

Genetic Engineering: Theory and Application.

What Will You Learn?

Course Content

Genetic Engineering: Theory and Application

Genetic Engineering: Theory and Application [Intro Video]

Lec 1: Cellular Structure (Part I)

Lec 2: Cellular Structure (Part II)

Lec 3: Cellular Structure (Part III)

Lec 4: Metabolic Reactions in Biological System

Lec 5: Growth Media For Different Expression System

Lec 6: Microbial Growth Kinetics

Lec 7: Isolation of a Gene Fragment (Part I)

Lec 8: Isolation of a Gene Fragment (Part II)

Lec 9: Isolation of a Gene Fragment (Part III)

Lec 10: Polymerase Chain Reaction

Lec 11: Molecular Tools for Cloning

Lec 12: Cloning Vectors- I

Lec 13: Cloning Vectors- II

Lec 14: DNA Delivery In Host- I

Lec 15: DNA Delivery In Host- II

Lec 16: Screening of Recombinant Clones

Lec 17: Protein Production in Host- I

Lec 18: Protein Production in Host- II

Lec 19: Protein Production in Host- III

Lec 20: Product Recovery from Host Cells

Lec 21: Basics of Chromatography (Part 1)

Lec 22: Basics of Chromatography (Part 2)

Lec 23: Ion-exchange Chromatography

Lec 24: Hydrophobic Interaction chromatography

Lec 25: Gel Filtration chromatography (Part 1)

Lec 26: Gel Filtration chromatography (Part 2)

Lec 27: Affinity Chromatography (Part 1)

Lec 28: Affinity Chromatography (Part 2)

Lec 29: Affinity Chromatography (Part 3)

Lec 30: Affinity Chromatography (Part 4)

Lec 31: Electrophoresis (Part 1)

Lec 32: Electrophoresis (Part 2)

Lec 33: Electrophoresis (Part 3)

Lec 34: Protein Sequencing

Lec 35: Spectroscopy (Part I)

Lec 36: Spectroscopy (Part II)

Lec 37: Biotechnology Applications (Part 1)

Lec 38: Biotechnology Applications (Part 2)

Lec 39: Biotechnology Applications (Part 3)

Lec 40: Summary and Conclusions (Part 1)

Lec 41: Summary and Conclusions (Part 2)

Student Ratings & Reviews