About Course
Essentials of Biomolecules: Nucleic Acids and Peptides.
The proposed course aims to provide the essentials of chemistry and biology of two very important classes of biomolecules: nucleic acids (DNA/RNA) and proteins.
The course allows us to decipher: how structural features are translated into biological functions; how highly organized and selective chemical reactions are adopted that allows DNA to replicate or dictates step-wise synthesis of a specific sequence of proteins; how organic chemistry tools in combination with enzymes were ingeniously applied to determine sequences of DNA and proteins and how chemical modifications could be done to mimic similar biological properties.
The course also includes modern techniques, the development of biomolecular probes as high-throughput detection of biomolecules, single nucleotide polymorphisms, and disease diagnosis. Overall, the course falls within the domain of organic chemistry and chemical biology.
INTENDED AUDIENCE: For chemistry, Biochemistry, and Biotechnology students
Course Content
Essentials of Biomolecules: Nucleic Acids and Peptides
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Lec 1: Importance of Biomolecules
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Lec 20: Transcription – The transfer of genetic information from DNA to mRNA
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Lec 21: Translation – The transfer of genetic information from mRNA to protein I
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Lec 22: Translation – The transfer of genetic information from mRNA to protein II
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Lec 23: Role of Ribosome in protein synthesis and the concept of codon
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Lec 24: Protein sequencing using Sanger’s and Edman’s degradation methods
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Lec 25: Mass spectroscopy and other sequencing methods for large proteins
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Lecture 26: Solution phase peptide synthesis : mechanism and end protection
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Lecture 27: Peptide coupling agents, Solid phase synthesis, peptide based therapeutics
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Lecture 28: Spectroscopic techniques
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Lecture 29: Spectrospic techniques -II and Purification technique-I of biomolecules
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Lecture 30: Purification techniques-II and Characterization techniques of biomolecules
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Lecture 31: Molecular probes : PNA and LNA-I
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Lecture 32: Molecular Probes: PNA and LNA – II
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Lecture 33: Carbohydrate chemistry-I: Introduction and Synthesis
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Lecture 34: Carbohydrate chemistry -II: Polysaccharides and its nanoparticles
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Lec 19: DNA repair
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Lec 18: Chemistry behind DNA damage and mutation
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Lec 17: Chemistry behind DNA damage and mutation
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Lec 2: DNA double helix: Chemical parameters
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Lec 3: DNA and Proteins
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Lec 4: Amino acids and Proteins
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Lec 5: Protein 3D structures, folding and denaturation
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Lec 6: Chemical synthesis pyrimidine nucleobases
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Lec 7: Chemical synthesis purine nucleobases, Prebiotic chemistry
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Lec 8: Synthesis of nucleosides
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Lec 9: Solid phase DNA synthesis
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Lec 10: Chemistry and Biology of DNA Replication
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Lec 11: Chemistry of Polymerase Chain Reaction
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Lec 12: Major components and steps involved in Polymerase chain reaction
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Lec 13: DNA sequencing: Sanger’s di-deoxy method
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Lec 14: DNA sequencing: Maxam- Gilbert method
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Lec 15: Numerical Problem – 1
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Lec 16: Sugar Chemistry
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Lecture 35: Carbohydrate chemistry -III: Synthesis of nanoparticles; Recap of all modules.
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