Essentials of Biomolecules: Nucleic Acids and Peptides

  • Course level: Beginner
  • Categories C-Science
  • Last Update 23/06/2021


Essentials of Biomolecules: Nucleic Acids and Peptides.

The proposed course aims to provide the essentials of chemistry and biology of two very important classes of biomolecules: nucleic acids (DNA/RNA) and proteins.

The course allows us to decipher: how structural features are translated into biological functions; how highly organized and selective chemical reactions are adopted that allows DNA to replicate or dictates step-wise synthesis of a specific sequence of proteins; how organic chemistry tools in combination with enzymes were ingeniously applied to determine sequences of DNA and proteins and how chemical modifications could be done to mimic similar biological properties.

The course also includes modern techniques, the development of biomolecular probes as high-throughput detection of biomolecules, single nucleotide polymorphisms, and disease diagnosis. Overall, the course falls within the domain of organic chemistry and chemical biology.

INTENDED AUDIENCE: For chemistry, Biochemistry, and Biotechnology students

What Will I Learn?

  • Week 1: Introduction, Nucleic acids, and proteins
  • Week 2: Synthesis of nucleobases, nucleotides, and oligonucleotides
  • Week 3: Solid-phase synthesis of oligonucleotides
  • Week 4: DNA replication, Polymerases, DNA sequencing, and PCR
  • Week 5: DNA damage, mutations, and cancer
  • Week 6: DNA to proteins: transcription, translation and genetic code
  • Week 7: Peptides, sequencing, and applications in therapeutics
  • Week 8: Solution phase and solid-phase peptide synthesis
  • Week 9: Expansion of genetic code: PNA, LNA, and molecular probes
  • Week 10: Modern techniques for biomolecules and disease diagnosis
  • Week 11: Structures and chemistry of sugars and carbohydrates
  • Week 12: Carbohydrate based polymers as biomolecular probes and therapeutics; conclusion

Topics for this course

35 Lessons

Essentials of Biomolecules: Nucleic Acids and Peptides

Lec 1: Importance of Biomolecules00:00:00
Lec 2: DNA double helix: Chemical parameters00:00:00
Lec 3: DNA and Proteins00:00:00
Lec 4: Amino acids and Proteins00:00:00
Lec 5: Protein 3D structures, folding and denaturation00:00:00
Lec 6: Chemical synthesis pyrimidine nucleobases00:00:00
Lec 7: Chemical synthesis purine nucleobases, Prebiotic chemistry00:00:00
Lec 8: Synthesis of nucleosides00:00:00
Lec 9: Solid phase DNA synthesis00:00:00
Lec 10: Chemistry and Biology of DNA Replication00:00:00
Lec 11: Chemistry of Polymerase Chain Reaction00:00:00
Lec 12: Major components and steps involved in Polymerase chain reaction00:00:00
Lec 13: DNA sequencing: Sanger’s di-deoxy method00:00:00
Lec 14: DNA sequencing: Maxam- Gilbert method00:00:00
Lec 15: Numerical Problem – 100:00:00
Lec 16: Sugar Chemistry00:00:00
Lec 17: Chemistry behind DNA damage and mutation00:00:00
Lec 18: Chemistry behind DNA damage and mutation00:00:00
Lec 19: DNA repair00:00:00
Lec 20: Transcription – The transfer of genetic information from DNA to mRNA00:00:00
Lec 21: Translation – The transfer of genetic information from mRNA to protein I00:00:00
Lec 22: Translation – The transfer of genetic information from mRNA to protein II00:00:00
Lec 23: Role of Ribosome in protein synthesis and the concept of codon00:00:00
Lec 24: Protein sequencing using Sanger’s and Edman’s degradation methods00:00:00
Lec 25: Mass spectroscopy and other sequencing methods for large proteins00:00:00
Lecture 26: Solution phase peptide synthesis : mechanism and end protection00:00:00
Lecture 27: Peptide coupling agents, Solid phase synthesis, peptide based therapeutics00:00:00
Lecture 28: Spectroscopic techniques00:00:00
Lecture 29: Spectrospic techniques -II and Purification technique-I of biomolecules00:00:00
Lecture 30: Purification techniques-II and Characterization techniques of biomolecules00:00:00
Lecture 31: Molecular probes : PNA and LNA-I00:00:00
Lecture 32: Molecular Probes: PNA and LNA – II00:00:00
Lecture 33: Carbohydrate chemistry-I: Introduction and Synthesis00:00:00
Lecture 34: Carbohydrate chemistry -II: Polysaccharides and its nanoparticles00:00:00
Lecture 35: Carbohydrate chemistry -III: Synthesis of nanoparticles; Recap of all modules.00:00:00

Enrolment validity: Lifetime


  • Basic knowledge in organic chemistry